serum free dmem (Thermo Fisher)

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Thermo Fisher serum free dmem
Serum Free Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dmem/Fluorescein/pmc13272512-283-8-10
Average 99 stars, based on 1 article reviews

serum free dmem - by Bioz Stars, 2026-10

99/100 stars

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Cell Culture:

Article Title: Multivalent adaptor networks generate nanoscale organisation within T cell signalling condensates
Article Snippet: .. HEK293T cells were cultured in DMEM (Gibco) media containing phenol red and 2mM glutamine, this was supplemented with 10% FBS, 1mM penicillin and streptomycin (all from Invitrogen). ..

Article Title: Quantitative analysis of autophagic flux reveals radiation-induced activation of SQSTM1-mediated degradation of protein aggregates and ER-phagy.
Article Snippet: Cell culture hTERT/RPE-1 cells (CRL-4000) were purchased from ATCC. .. The cells were cultured in DMEM (high-glucose, GlutaMAX supplement and HEPES; Gibco, #10564029) supplemented with 10% fetal bovine serum (FBS) (Serana Europe, #S-FBS-MX-015) and 1× penicillin–streptomycin (Nacalai Tesque, #09367–34) in a humidified atmosphere of 5% CO2 at 37◦C. .. HaloTag7-mGFP, HaloTag7-mGFP-KDEL or pSu9-HaloTag7-mGFP stable expression cell lines were generated by transfection using Xfect Transfection Reagent (Clontech, #631317), followed by long-term drug selection.

Article Title: Conformational diversity of collided ribosomes determines stress signaling
Article Snippet: .. Adherent cells were cultured in DMEM (high glucose, L-glutamine, phenol red, sodium pyruvate - Thermo Fisher #11995073) supplemented with 10% FBS (Thermo Fisher #26140079). ..

Article Title: Antimicrobial photodynamic therapy with Ligularia fischeri extract and red light improves the restoration of Staphylococcus aureus-infected wounds in BALB/c mice
Article Snippet: .. Cells were seeded in a 96-well culture plate at a concentration of 2 × 104 cells/well and cultured for 24 h. After removing the existing medium from each well, the cultured cells were treated with 100 μL of DMEM (without phenol red, Thermo Fisher Scientific) containing the prepared LF (0–1000 μg/mL), and incubated for 30 min prior to RL irradiation, followed by RL irradiation (660 nm, 120 W/m2, 15 min; light dose: 10.8 J/cm2) or none, and then cultured for 24 h. After incubation, the medium was removed from each well, and 100 mL of MTT solution (0.5 mg/mL, Sigma-Aldrich) prepared using DMEM was added and cells were cultured for 1 h. Subsequently, the MTT solution was removed from each well, and then 100 mL of DMSO was added to dissolve the formazan crystals, and then the absorbance value was measured at 560 nm using an Infinite M1000 microplate reader. .. RAW 264.7 cells, a murine macrophage cell line, were obtained from ATCC and maintained in DMEM supplemented with 10% (v/ v) fetal bovine serum and 1% (v/v) penicillin–streptomycin in a humidified incubator with 5% CO2 at 37 °C.

Concentration Assay:

Incubation:

Article Title: Immunomodulating effects of herbal cough medication - in vitro screening in a TLR7- and TLR8-mediated inflammatory model.
Article Snippet: For cultivation, culture flasks were coated with collagen (60 μg/ml, Sigma-Aldrich, St. Louis, United States). .. AR TIC LE IN PR ES S ARTICLE IN PRESS Scientific) and incubated for 20 min at 37°C in DMEM (w/o phenol red, Gibco by Thermo Fischer Scientific) containing 5% water-soluble tetrazolium 1 (WST-1) solution (Roche, Indianapolis, USA). .. Cells were then treated with the indicated dilutions of the herbal medications, staurosporine (10 μM; inhibition control, dissolved in DMSO, Sigma Aldrich) or the corresponding solvent controls.

Irradiation:

MTT Assay:

Chromatography:

Article Title: Hypothesis-free evaluation of circulating metabolome provides cell-specific insights regarding the role of energy substrate availability in amyotrophic lateral sclerosis.
Article Snippet: .. Liquid chromatography-mass spectrometry analysis On day 6 of differentiation cell media was changed to DMEM (no phenol red, glucose, Lglutamine; Gibco A14430-01), supplemented with 10% FBS, 5mM D-glucose or 5mM DFructose and 0.3nM L-Glutamine. ..

Blocking Assay:

Article Title: Reversible superdeformability of hiPSC epithelial cortinoids.
Article Snippet: .. Permeabilization and blocking prior to immunostaining were performed at room temperature for 2 h in a solution of DMEM (without red phenol, 11054020, Gibco), Triton X100 0.1% (A4975, AppliChem Panreac), Tween 0.2% (Sigma P1397) and FBS 10% (Capricorn Scientfic FBS-16A). .. To stain the baso-lateral edges of the cells in the epithelium (Fig. 3A), primary antibody epCAM (EpCAM Monoclonal MA512436, Thermofisher) was used at 1:100 dilution, followed by a conjugation with a secondary antibody (Alexa-fluor 647 A32787, Thermofisher) at 1:500 dilution.

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